Detection modalities. Instructions on the proper use of the SpectraMax M5 Plate Reader. After the plate is read, data will appear in the plate. 3: The cuvette chamber. Performing an enzyme dilution series verifies the enzyme and assay performance and allows calculation of the EC 80 concentration of enzyme that will be used for inhibitor screening assays. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Similar settings will work for other SpectraMax readers with fluorescence detection. 72 MB. 0 out of 5 $ 16,499. Select the Driver tab. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Cell-based experiments In the first experiment, we prepared a dilutionThe integration time suggested is 5-10 sec. Shake. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. 12. Opens in a new window or tab. 2. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 1321N1, a human astrocytoma cell line, expresses. Step 4. Dynamic Range. Spectramax i3x multi-mode microplate readers from molecular devices llc Spectramax m5 microplate reader (molecular devices) Molecular devices spectramax m5 microplate reader, sn: mv06308 100-240v Molecular devices spectramax i3x 使用される 販売のために 価格（税込） #9298540, 2017The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. 2 with 1000 cells. The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 304 signal level saturation. Learn how you can utilize microplate readers to drive landmark discoveries. The reader temperature was set to 37 Celsius and most the the liquid in the wells of the plate had evaporated when I went to dispose of it. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Pathcheck Pathlength Measurement Technology. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 95. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Our absorbance plate readers feature our PathCheck Sensor technology and. SpectraMax Spectrofluorometers. Figure 1. 3: The cuvette chamber. View the Molecular Devices website. Be sure to insert the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. com 10. 1. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Optics. Add to Cart. This. optical beams than older wide-beam plate readers, are much more vulnerable to spurious readings due to dust. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Note: In this user guide, all references to SpectraMax Multi-Mode. 200 ‚ 1000 nm. When a feature or capability applies to only certain readers, this exception is noted. SpectraMax M5 reader SpectraMax M5e. No accessories are needed and SoftMax® Pro Software. 12. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Spectramax M5. SpectraTest ABS2 Absorbance Validation Package performance tests. PURPOSE. 18 x 14 x 8 ins. Molecular Devices Spectramax M5 Microplate Reader. D 49 7. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. In this product video, see how the SpectraMax M5 quickly reads samples. These robust readers have been placed in labs from. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. The SpectraMax M5 is a multi-detection, monochromator-based reader with both topand bottom-read capability. The following measurements are automatically made by SoftMax Pro Software: baseline noise, dark noise, optical alignment, wavelength accuracy, photometric linearity and wavelength precision. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Table of Contents. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. The SpectraMax® ABS Plus Microplate Reader can run . ”The SpectraMax 190 reader can be integrated with Molecular . Designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 5 pM (0. Prepare your iD series instrument for shipment. 标准功. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. In an analogous manner, the same sample names were assigned to a CuvetteSet section. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Also refer to this Multimode Reader Comparison Chart. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. There is currently no charge for using this plate reader. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 0% ± 0. Uncontrolled Copy for Training and Reference Purposes Only . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. HTRF-compatible SpectraMax Multi-Mode Readers. 316 grating motor driver faulty. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The cuvette must be in place when you read the plate. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. 95. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Description 1. SpectraMax® Microplate Readers and SoftMax® Pro Software. Shop now. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. With optimized reagents and the industry-leading. com. 0 out of 5 $ 17,999 $ 16,995. Optical absorbance was measured at 734 nm on a SpectraMax M5. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. techsupport. 0% ± 0. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Page 28 Chapter 5: Read Modes and Read Types 1. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Prepare your i3/i3x instrument for shipment. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. 00. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 1. Features. Microplate Readers include the M3, M4,. 40266 Email: [email protected] only) SpectraMax M-series Microplate readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. #3635 or Greiner cat. 7°C and the bottom to 36. 6 for further experiment, so I'm confused which i would choose for OD value determination. I accidentally left a plate in my lab's M5 SpectraMax plate reader overnight. 87. Last lab had ours for years and never had a problem with it. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. $6,649. 8. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. After the plate is read, data will appear in the plate section: 11. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. Consider these features as well as all of the intended applications when choosing an appropriate luminescence reader for the lab. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. columns 1, 3, 5, 7, 9 and 11 were seeded with 200,000 mock-transfected cells per well. I have been using the SpectraMax M5 since 2011. Supported Plates and Cuvettes. SpectraMax M5 Multi-Mode Microplate Reader. 40266 Email: drugdiscoverytech@lifetech. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. Plate reader instructions Computer. Description 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. readers, the vertical light beam results in a pathlength that depends on the vol-. 9. Download PDF. Read one sample or up to 384 in a single plate read using any standard. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. 3752 in as per SBS dimensions. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Description. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. M5 E -HTRF Readers. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A 5 1. Figure 10: The Plate section Template Editor, showing the series set up. FlexStation. 12. SpectraMax M5e Multi-Mode Microplate Reader. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. Plate section without PathCheck applied. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Supplier: Molecular Devices. Pick the best speed, sensitivity, and price for your needs. A standard curve was plotted using a 4-parametric. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 58% for the detection module and 2. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Compact and tunable absorbance microplate readers DATA SHEET. 6: bioNova científica, s. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Description. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. We would like to show you a description here but the site won’t allow us. ". Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 2. no. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Opens in a new window or tab. Self Service Available. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Model: SpectraMax M5. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The chamber. The SpectraMax ® i3x. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The dedicated luminescence PMT has extremely low background counts and superior performance in the blue wavelengths. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. A dialog box will appear, requesting you to confirm that the read is a pre-read. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. 6% for the on-board optics (monochromators). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. FlexStation II 384. Shop all used microplate readers and parts from a variety of manufacturers. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created Date:1) Select the correct plate and the wells that you want to measure. For more sample throughput, the system canThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. At NanoRacks, the challenge for the engineers will be to modify the plate reader in order for the unit to operate comfortably in the zero-gravity environment of the space station. Label: provides information. SpectraMax i3 reader settings for IMAP PDE assays. 0200-0729. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The plate was incubated at room temperature for one hour. ScanLater Western Blot Detection Cartridge. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper. 40266 Email: drugdiscoverytech@lifetech. For the BioVision cytotoxicity kit, 100 µL of culture medium was removed from each treated well and transferred to a solid white plate. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. +44 118 944 8000. Update Driver. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. iD5. 314 low lamp level warning. Gemini XPS. EarlyTox Caspase-3/7 R110 Assay. 03 in x 3. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or We use Spectramax M5 microplate reader in our lab. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Description The Onboard Microprocessor. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader,. Uncontrolled Copy for Training and Reference Purposes Only . Page 1 of 8 . 0 out of 5 $ 9,995 $ 9,495. Human macrophages choreograph tissue development. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. Features. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Considerations for readers include the measurement wavelength range, microwell plate capacity, throughput, detection methods, and a filter vs monochromator-based optical system. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. ". Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSetup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 7. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 1. 0 out of 5 $ 3,799. In the lower half of the window, left side, there is a box labeled reader. What do I do now? In our tests, the Greiner Bio-One Fluotrac™ 200 Microplate has shown lower background. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. BENEFITS • No filters needed • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer. Catalog Number: M5. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. We use Spectramax M5 microplate reader in our lab. Thank you!. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Label-free detection. 4. Figure 1. It also integrates with the. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The chamber. Description 1. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Microplate readers’ standard detection modes. begin reading microplates with seven mouse clicks. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 4. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. . S. SpectraMax M5 Multi-Mode Microplate Reader. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 2. Compact and tunable absorbance microplate readers DATA SHEET. Italic and boldface type are used for emphasis. Supersedes 1. SpectraMax M3, M4, M5, and M5 e. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. SpectraMax Glo Steady-Luc Reporter Assay Kit. Description. The following document is. 2018. l. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Please refer to the Obsolete Microplate Reader Models for more information. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Table of Contents. Put the plate in the SPECTRAmax PLUS drawer, then click the Read but-ton in the Plate section’s Status Bar. Click SoftMax Pro Software User Guide. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. Trends in Immunology. 010 OD 2. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated. Spectramax M5. 0 out of 5 $ 15,999. ZERO BIAS - scores, article reviews, protocol conditions and morePage 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Choose from a three- (M3), four- (M4), or96-Well Plate, Normal Read Speed: 0. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Turn the reader on and secure the USB cable. However, when a specific probe is not available, or it causes off-target effects on the. Pathcheck Pathlength Measurement Technology. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Plate Shaking Linear, Orbital Slow, and Orbital Fast.